Efficient utilization of licorice root by alkaline extraction.

نویسندگان

  • Hirokazu Ohno
  • Shozo Miyoshi
  • Daisuke Araho
  • Taisei Kanamoto
  • Shigemi Terakubo
  • Hideki Nakashima
  • Tadashi Tsuda
  • Katsuyoshi Sunaga
  • Shigeru Amano
  • Emika Ohkoshi
  • Hiroshi Sakagami
  • Kazue Satoh
  • Masaji Yamamoto
چکیده

Compared to studies of water extracts of plants, those utilising alkaline extracts are limited. Both water and alkaline extracts from licorice root were compared regarding their biological activities. Licorice root was successively extracted first with water or alkaline solution (pH 9 or 12), and the alkaline (pH 12.0) extract was further separated into 50% ethanol-soluble and -insoluble fractions. Viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Antibacterial activity against Porphyromonas gingivalis 381 was determined by turbidity assay. Cytochrome P-450 (CYP)3A4 activity was measured by β-hydroxylation of testosterone using human recombinant CYP3A4. Radical intensity of superoxide and hydroxyl radicals was determined by electron spin resonance spectroscopy. Alkaline extraction yielded slightly higher amounts of dried materials compared to water extraction. Alkaline extract showed higher anti-HIV and antibacterial activities, and similar magnitudes of CYP3A4 inhibitory and superoxide and hydroxyl radical-scavenging activities, compared to water extract. When alkaline extract was fractionated by 50% ethanol, anti-HIV activity was recovered from the insoluble fraction representing approximately 3% of the alkaline extract, whereas antibacterial activity was concentrated in the soluble fraction rich in glycyrrhizid acid, flavanones and chalcones. All extracts and sub-fractions led to bimodal hormetic dose-response (maximum hormetic response=238%) on the bacterial growth. The present study demonstrated the superiority of alkaline extraction over water extraction for preparing anti-HIV and antibacterial agents at higher yield from licorice root.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

In Vitro Comparative Evaluation of the Antibacterial Efficacy of Licorice Aqueous Root Extract (Glycyrrhiza Glabra) and Chlorhexidine against Lactobacillus Acidophilus

Background and Aim: Oral infections and dental caries are considered as two serious public health problems, which inflict a costly burden on health care services worldwide, especially in the developing countries. The aim of this study was to evaluate the antibacterial activity of Iranian Licorice aqueous root extract on Lactobacillus Acidophilus in comparison with Chlorhexidine. Material...

متن کامل

The study of analgesic effect of licorice root extract in rat

Although non-steroidal anti-inflammatory drugs (NSAIDs) and opioid compounds are widely used in the releif of pain, they are not always useful due to their side effects. It seems necessary to search for newer analgesic compounds. Therefore, we decided to study the analgesic effects of this plant. For extraction, percolation method was used and different weights of dried extract were dissolved i...

متن کامل

The study of analgesic effect of licorice root extract in rat

Although non-steroidal anti-inflammatory drugs (NSAIDs) and opioid compounds are widely used in the releif of pain, they are not always useful due to their side effects. It seems necessary to search for newer analgesic compounds. Therefore, we decided to study the analgesic effects of this plant. For extraction, percolation method was used and different weights of dried extract were dissolved i...

متن کامل

Optimization of Ultrasound Assisted Extraction of Isoliquiritigenin from Licorice by Response Surface Methodology

Isoliquiritigenin (ILG) of licorice is a potential cancer chemopreventive agent. In this paper, response surface methodology (RSM) integrating with Box-Behnken design (BBD) was firstly employed to optimize the ILG extraction, which based on the single-factor experiments. According to analysis, the effect of extraction time and ethanol concentration on yields was significantly. The optimum value...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • In vivo

دوره 28 5  شماره 

صفحات  -

تاریخ انتشار 2014